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Study of the occurrence of colistin in soil
Veldamonová, Aneta ; Zlámalová Gargošová, Helena (referee) ; Mravcová, Ludmila (advisor)
Colistin is a polypeptide antibiotic used as a "last resort" effective against Gram-negative bacteria. However, increase in its consumption in veterinary medicine in the last 30 years has led to the development of bacterial resistance even to colistin. The application of slurry to soil containing unmetabolized colistin and resistant bacteria poses a risk, because the resistance of bacteria to colistin can be further developed and spread to other components of the environment. Therefore, this work was focused on the creation and optimization of extraction and analytical methods for soil samples containing colistin. Colistin was detected by liquid chromatography in connection with mass spectrometry (HPLC/MS and UPLC/MS/MS). Many extraction solutions have been tested in connection with ultrasonic extraction and purification on various SPE columns, yet colistin has not been successfully extracted from the soil. Colistin was successfully detected only in the slurry extract. The reason why colistin was not detected in soil extracts could be the sorption of colistin on soil organic matter or the formation of complexes of colistin with humic substances.
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Analysis of pesticides in the environment using the QuEChERS method
Vašinková, Alena ; Řezáčová, Veronika (referee) ; Mravcová, Ludmila (advisor)
This thesis focuses on pesticides in the environment, more specifically in cereals. Pesticides were monitored in various samples of cereals (barley, rye, oats, and wheat) within the thesis. The monitored substances were isolated in selected samples and concentrated using the QUECHERS method. Gas and liquid chromatography were used to determine pesticides. The theoretical part consists of the common types of pesticides, divided by the ease with which these substances into food and their impact on humans and environment. It also mentions current health protection legislation and environmental legislation.
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Analysis of pesticides in the environment using the QuEChERS method
Vašinková, Alena ; Řezáčová, Veronika (referee) ; Mravcová, Ludmila (advisor)
This thesis focuses on pesticides in the environment, more specifically in cereals. Pesticides were monitored in various samples of cereals (barley, rye, oats, and wheat) within the thesis. The monitored substances were isolated in selected samples and concentrated using the QUECHERS method. Gas and liquid chromatography were used to determine pesticides. The theoretical part consists of the common types of pesticides, divided by the ease with which these substances into food and their impact on humans and environment. It also mentions current health protection legislation and environmental legislation.
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Study of the occurrence of colistin in soil
Veldamonová, Aneta ; Zlámalová Gargošová, Helena (referee) ; Mravcová, Ludmila (advisor)
Colistin is a polypeptide antibiotic used as a "last resort" effective against Gram-negative bacteria. However, increase in its consumption in veterinary medicine in the last 30 years has led to the development of bacterial resistance even to colistin. The application of slurry to soil containing unmetabolized colistin and resistant bacteria poses a risk, because the resistance of bacteria to colistin can be further developed and spread to other components of the environment. Therefore, this work was focused on the creation and optimization of extraction and analytical methods for soil samples containing colistin. Colistin was detected by liquid chromatography in connection with mass spectrometry (HPLC/MS and UPLC/MS/MS). Many extraction solutions have been tested in connection with ultrasonic extraction and purification on various SPE columns, yet colistin has not been successfully extracted from the soil. Colistin was successfully detected only in the slurry extract. The reason why colistin was not detected in soil extracts could be the sorption of colistin on soil organic matter or the formation of complexes of colistin with humic substances.
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